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Tri-lineage differentiation testing is one of the important indicators for identifying mesenchymal stem cells (MSCs). By inducing MSCs to differentiate into osteoblasts, chondrocytes, and adipocytes, the application potential of these cells in tissue engineering and regenerative medicine can be evaluated.
This product is specifically designed for tri-lineage differentiation testing of MSCs derived from human umbilical cord and adipose tissue in a serum-free culture system. It can also be used for MSCs derived from serum-containing systems, serum replacement systems, or induced pluripotent stem cells (iPSCs).
Osteogenic Differentiation
Chondrogenic Differentiation
Adipogenic Differentiation
Performance Demonstration
Differentiation Effect— Osteogenic Differentiation
Using Yocon Biotech's GMP-grade serum-free mesenchymal stem cell (MSC) medium (product codes NC0106+NC0106.S) to culture P5 generation MSCs for osteogenic differentiation, calcium nodules can be observed after 14 days, with a calcium nodule coverage rate exceeding 90% at 21 days.
Differentiation Effect — Chondrogenic Differentiation
Using Yocon Biotech's GMP-grade serum-free mesenchymal stem cell (MSC) medium (product codes NC0106+NC0106.S) to culture P5 generation MSCs for chondrogenic differentiation, visible blue staining with Alcian Blue can be observed approximately 20 days after induction of differentiation.
Differentiation Effect — Adipogenic Differentiation
Using Yocon Biotech's GMP-grade serum-free mesenchymal stem cell (MSC) medium (product codes NC0106+NC0106.S) to culture P5 generation MSCs for adipogenic differentiation, preliminary observation of lipid droplet formation can be made on day 8, and staining reveals a significant number of lipid droplets by day 12.
Osteogenic Differentiation
Chondrogenic Differentiation
Adipogenic Differentiation
Performance Demonstration
Differentiation Effect— Osteogenic Differentiation
Using Yocon Biotech's GMP-grade serum-free mesenchymal stem cell (MSC) medium (product codes NC0106+NC0106.S) to culture P5 generation MSCs for osteogenic differentiation, calcium nodules can be observed after 14 days, with a calcium nodule coverage rate exceeding 90% at 21 days.
Differentiation Effect — Chondrogenic Differentiation
Using Yocon Biotech's GMP-grade serum-free mesenchymal stem cell (MSC) medium (product codes NC0106+NC0106.S) to culture P5 generation MSCs for chondrogenic differentiation, visible blue staining with Alcian Blue can be observed approximately 20 days after induction of differentiation.
Differentiation Effect — Adipogenic Differentiation
Using Yocon Biotech's GMP-grade serum-free mesenchymal stem cell (MSC) medium (product codes NC0106+NC0106.S) to culture P5 generation MSCs for adipogenic differentiation, preliminary observation of lipid droplet formation can be made on day 8, and staining reveals a significant number of lipid droplets by day 12.
