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Artificial recombinant system, without human or animal derived components, and the endotoxin in the products can be controlled at <0.25EU/mL. It has been filed with the FDA DMF, with the filing No. of 37790, which greatly reduces the workload and difficulty in submitting the application for stem cell drugs.
The conventional digestion time is 2 min, and the limit digestion time is up to 15 min, which has solved the problem that the digestion time and digestion strength are difficult to control when the cells are digested by traditional trypsin. After digestion, there is no need to stop.
The product can be obtained by diluting the supernatant of cell culture, which can greatly reduce the use cost.
Product Description
| Product name | Item No. | Specification | Storage conditions |
| Gentle digestive enzymes for stem cells | NC1004.1 | 500 mL/bottle | At 2-8° C, stored at places protect from light for 12 months |
| NC1004.2 | 100 mL/bottle |
Product Application
1. It is used for the storage of immune cells and stem cells.
2. It is applied in cell preservation centers for the long - term preservation of cell lines.
3. It is utilized in scientific research institutions and universities for the cryopreservation of cell lines.
4. It is used for the preservation of cell samples in hospital sample banks.
Product Principle
The serum - free cell cryopreservation solution contains various cryoprotectant components. Some cryoprotectants can easily penetrate cells, preventing the formation of ice crystals from water molecules inside the cells and thus avoiding cell damage. Meanwhile, some other cryoprotectants cannot penetrate cells, but they can preferentially bind to water molecules outside the cells before ice crystal formation. This reduces the electrolyte concentration of the extracellular solution and decreases the number of cations entering the cells. Our company's serum - free cell cryopreservation solution is a combination of multiple cryoprotectants, providing dual - protection both inside and outside the cells, which greatly improves the cell resuscitation survival rate.
Main Components
Amino acids, vitamins, inorganic salts, albumin, cryoprotectants.
Performance comparison with conventional trypsin
* The above data was the corresponding data obtained from P4 cells obtained by inoculating P2 seed cells of MSC of umbilical cord origin in T25 culture flasks with an inoculation density of 8,000 cells/cm² and subculturing the P3 cells obtained by digestion after 72 h.
Product Description
| Product name | Item No. | Specification | Storage conditions |
| Gentle digestive enzymes for stem cells | NC1004.1 | 500 mL/bottle | At 2-8° C, stored at places protect from light for 12 months |
| NC1004.2 | 100 mL/bottle |
Product Application
1. It is used for the storage of immune cells and stem cells.
2. It is applied in cell preservation centers for the long - term preservation of cell lines.
3. It is utilized in scientific research institutions and universities for the cryopreservation of cell lines.
4. It is used for the preservation of cell samples in hospital sample banks.
Product Principle
The serum - free cell cryopreservation solution contains various cryoprotectant components. Some cryoprotectants can easily penetrate cells, preventing the formation of ice crystals from water molecules inside the cells and thus avoiding cell damage. Meanwhile, some other cryoprotectants cannot penetrate cells, but they can preferentially bind to water molecules outside the cells before ice crystal formation. This reduces the electrolyte concentration of the extracellular solution and decreases the number of cations entering the cells. Our company's serum - free cell cryopreservation solution is a combination of multiple cryoprotectants, providing dual - protection both inside and outside the cells, which greatly improves the cell resuscitation survival rate.
Main Components
Amino acids, vitamins, inorganic salts, albumin, cryoprotectants.
Performance comparison with conventional trypsin
* The above data was the corresponding data obtained from P4 cells obtained by inoculating P2 seed cells of MSC of umbilical cord origin in T25 culture flasks with an inoculation density of 8,000 cells/cm² and subculturing the P3 cells obtained by digestion after 72 h.
