Adipose-Derived Mesenchymal Stem Cell Serum-Free Medium
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Adipose-Derived Mesenchymal Stem Cell Serum-Free Medium

  • The serum-free medium of mesenchymal stem cells specially designed for adipose-derived cells can harvest 4.5x10⁶ primary cells from 10 mL of adipose tissue, and can be continuously subcultured to P20 without changing the medium.
 
  • A truly serum-free pure factor system, with no human-derived or animal-derived components, for which biocompatibility and toxicity experiments have been specifically conducted to guarantee the safety of the culture medium, and with more focuses on safety.

 
Availability:

Product Description

Product name    Item No. Specification Purpose
Base of serum-free medium for mesenchymal  stem cells NC0103 500 mL/bottle  5 mL of NC0104.S added to 500 mL of NC0103 basic  medium can be used for primary isolation and  subculture of adipose-derived mesenchymal stem  cells
Base of serum-free medium for mesenchymal  stem cells NC0104.S 5 mL/bottle
Adipose tissue digestive enzymes NC1005 100 mL/bottle Specially used for the digestion of adipose tissues to  separate primary

Purpose and Description

This medium can be used for the proliferation and culture of human mesenchymal stem cells from various  sources, and can also maintain their multi - directional differentiation potential, such as bone marrow (BM -  hMSC) and umbilical cord (UCM - hMSC). It is not applicable to the isolation of primary umbilical cord cells by  tissue methods. No serum or serum substitutes need to be added when using this product. This product has a clear chemical composition, without added serum or serum substitutes (platelet lysates),  and without any animal - derived components. The batch - to - batch variation of the product is small, and all  raw materials meet GMP standards. It is more suitable for clinical research purposes. 

Product Design Principle 

Based on basic media such as DMEM, MEM, and M199, various amino acids, vitamins, inorganic salts, human  albumin, transferrin, insulin, and trace elements are added. It can not only isolate primary MSCs but also  culture MSCs in the later stage, maintain the normal morphology and phenotype of cells, and avoid certain  components in serum or serum substitutes that can promote stem cell differentiation. During cell passage, it  can maintain the undifferentiated state of stem cells. 

Main Components

Amino acids, vitamins, inorganic salts, human albumin, transferrin, insulin, trace elements, etc.

Data of Continuous Subculturing

44

*The above data was obtained from serum free medium and adipose tissue digestive enzymes by Yocon  Biology adipose separation technique. Different adipose samples and different separation techniques may  lead to significant differences in results. 



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