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Product Description
Product name | Item No. | Specification | Purpose |
Base of serum-free medium for mesenchymal stem cells | NC0103 | 500 mL/bottle | 5 mL of NC0104.S added to 500 mL of NC0103 basic medium can be used for primary isolation and subculture of adipose-derived mesenchymal stem cells |
Base of serum-free medium for mesenchymal stem cells | NC0104.S | 5 mL/bottle | |
Adipose tissue digestive enzymes | NC1005 | 100 mL/bottle | Specially used for the digestion of adipose tissues to separate primary |
Purpose and Description
This medium can be used for the proliferation and culture of human mesenchymal stem cells from various sources, and can also maintain their multi - directional differentiation potential, such as bone marrow (BM - hMSC) and umbilical cord (UCM - hMSC). It is not applicable to the isolation of primary umbilical cord cells by tissue methods. No serum or serum substitutes need to be added when using this product. This product has a clear chemical composition, without added serum or serum substitutes (platelet lysates), and without any animal - derived components. The batch - to - batch variation of the product is small, and all raw materials meet GMP standards. It is more suitable for clinical research purposes.
Product Design Principle
Based on basic media such as DMEM, MEM, and M199, various amino acids, vitamins, inorganic salts, human albumin, transferrin, insulin, and trace elements are added. It can not only isolate primary MSCs but also culture MSCs in the later stage, maintain the normal morphology and phenotype of cells, and avoid certain components in serum or serum substitutes that can promote stem cell differentiation. During cell passage, it can maintain the undifferentiated state of stem cells.
Main Components
Amino acids, vitamins, inorganic salts, human albumin, transferrin, insulin, trace elements, etc.
Data of Continuous Subculturing
*The above data was obtained from serum free medium and adipose tissue digestive enzymes by Yocon Biology adipose separation technique. Different adipose samples and different separation techniques may lead to significant differences in results.
Product Description
Product name | Item No. | Specification | Purpose |
Base of serum-free medium for mesenchymal stem cells | NC0103 | 500 mL/bottle | 5 mL of NC0104.S added to 500 mL of NC0103 basic medium can be used for primary isolation and subculture of adipose-derived mesenchymal stem cells |
Base of serum-free medium for mesenchymal stem cells | NC0104.S | 5 mL/bottle | |
Adipose tissue digestive enzymes | NC1005 | 100 mL/bottle | Specially used for the digestion of adipose tissues to separate primary |
Purpose and Description
This medium can be used for the proliferation and culture of human mesenchymal stem cells from various sources, and can also maintain their multi - directional differentiation potential, such as bone marrow (BM - hMSC) and umbilical cord (UCM - hMSC). It is not applicable to the isolation of primary umbilical cord cells by tissue methods. No serum or serum substitutes need to be added when using this product. This product has a clear chemical composition, without added serum or serum substitutes (platelet lysates), and without any animal - derived components. The batch - to - batch variation of the product is small, and all raw materials meet GMP standards. It is more suitable for clinical research purposes.
Product Design Principle
Based on basic media such as DMEM, MEM, and M199, various amino acids, vitamins, inorganic salts, human albumin, transferrin, insulin, and trace elements are added. It can not only isolate primary MSCs but also culture MSCs in the later stage, maintain the normal morphology and phenotype of cells, and avoid certain components in serum or serum substitutes that can promote stem cell differentiation. During cell passage, it can maintain the undifferentiated state of stem cells.
Main Components
Amino acids, vitamins, inorganic salts, human albumin, transferrin, insulin, trace elements, etc.
Data of Continuous Subculturing
*The above data was obtained from serum free medium and adipose tissue digestive enzymes by Yocon Biology adipose separation technique. Different adipose samples and different separation techniques may lead to significant differences in results.