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Performance advantages
It is used to induce and expand human fresh and cryopreserved peripheral blood mononuclear cells (PBMCs) into γδ T cells in vitro. After 14 to 16 days of culture, the PBMCs can achieve a cell expansion of more than 200 times and a positive rate of over 85%.
It is induced by pure factors without the need for feeder layer cells. The culture method is simple and easy to implement, and the cell states are basically uniform. According to the recommended process for adding culture medium, there is no need to perform cell counting.
When paired with the newly upgraded NK5.0 basal medium, it significantly enhances the continuous expansion ability of the cells.
Scope of Application
| Product Name | Cat. No. | Product Use | Product Specification | Storage Temperature |
| NK Cell Serum-Free Medium | NC0102 | For in vitro culture of γδT cells | 2 bottles, 1L/ bottle | 2~8℃ |
| γδT Cell Induction and Expansion Kit Set | AN0110 | YC00T: Added during initial culture | 1 vial， 200μL/ vial | -20℃ |
| YC005: Added to the culture medium | 2 vials, lyophilized powder |
Case Presentation
Sample 1: A fresh peripheral blood mononuclear cell sample from a 25-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 9 billion cells were harvested, and the positive rate was 90.01%.
Sample 2: A fresh peripheral blood mononuclear cell sample from a 38-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.1 billion cells were harvested, and the positive rate was 92.66%.
Sample 3: A cryopreserved peripheral blood mononuclear cell sample from a 35-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.2 billion cells were harvested, and the positive rate was 90.29%.
Performance advantages
It is used to induce and expand human fresh and cryopreserved peripheral blood mononuclear cells (PBMCs) into γδ T cells in vitro. After 14 to 16 days of culture, the PBMCs can achieve a cell expansion of more than 200 times and a positive rate of over 85%.
It is induced by pure factors without the need for feeder layer cells. The culture method is simple and easy to implement, and the cell states are basically uniform. According to the recommended process for adding culture medium, there is no need to perform cell counting.
When paired with the newly upgraded NK5.0 basal medium, it significantly enhances the continuous expansion ability of the cells.
Scope of Application
| Product Name | Cat. No. | Product Use | Product Specification | Storage Temperature |
| NK Cell Serum-Free Medium | NC0102 | For in vitro culture of γδT cells | 2 bottles, 1L/ bottle | 2~8℃ |
| γδT Cell Induction and Expansion Kit Set | AN0110 | YC00T: Added during initial culture | 1 vial， 200μL/ vial | -20℃ |
| YC005: Added to the culture medium | 2 vials, lyophilized powder |
Case Presentation
Sample 1: A fresh peripheral blood mononuclear cell sample from a 25-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 9 billion cells were harvested, and the positive rate was 90.01%.
Sample 2: A fresh peripheral blood mononuclear cell sample from a 38-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.1 billion cells were harvested, and the positive rate was 92.66%.
Sample 3: A cryopreserved peripheral blood mononuclear cell sample from a 35-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.2 billion cells were harvested, and the positive rate was 90.29%.
