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The high-performance version can obtain more than 180 times of cell expansion and more than 85% of positive rate after 14~16 days of culturing. It can be specifically used for the activation and culture of NK cells in unconventional cord blood or peripheral blood samples, such as blood samples with a long-distance transportation time of more than 8 hours or unknown cryopreservation quality, and supports the culture protocol without the addition of autologous plasma.
Induction with pure factors, with no trophoblastic cells, simple and feasible culture method, basically uniform cell state, fed-batch of solutions according to the recommended process, without the need for cell counting. When combined with the newly upgraded NK5.0 basic culture medium, it significantly improves the continuous expansion ability of cells after 7 consecutive days of cultivation, and the continuous support ability of cells that expand from 2 L system to 3 L or even 4 L system after 14 days.
It can be paired with the research-grade basal medium (NC0102) or the chemically-defined basal medium (NC0102.H). The basal medium has been filed with the US FDA DMF with the filing No. of 37874.
Version Selection
Version | Application Scope | Product item number | |
Inoculation blood volume | Item No. | ||
Standard Version | 1. Sample initial positivity rate above 10%; 2. No specific requirement for extremely high positivity rate (above 90%); 3. Can accept special samples with positivity rate below 70%. | 30mL | NC0102+AN0107 |
50mL | NC0102+AN0107-2 | ||
High-Performance Version | 1. Samples with low initial positivity rate or not tested; 2. High demand for extremely high positivity rate (above 90%); 3. Pursuit of extremely high success rate and fault tolerance. As well as the following special circumstances: 1. Samples transported for a long time (transport time exceeding 8 hours); 2. Frozen samples that used non-YOCON mononuclear cell cryopreservation solution; 3. No autologous plasma available for addition. | 30mL | NC0102.F +NC0102+AN0104 |
50mL | NC0102.F +NC0102+AN0104-2 | ||
*All the above-mentioned basal media can be combined with the research-grade serum-free NK cell culture medium (NC0102) or the chemically defined serum-free NK cell culture medium (NC0102.H)
Product Advantages
1. For normal blood samples, the standard version can achieve over 180 - fold cell expansion and a positive rate of over 50% after 14 - 16 days of culture. The high - performance version can achieve over 200 - fold cell expansion and a positive rate of over 85% after 14 - 16 days of culture. The high - performance version can be used for the activation and culture of NK cells in unconventional cord blood or peripheral blood samples, such as blood samples with long - distance transportation time exceeding 8 hours or those with unknown cryopreservation quality, and it supports the culture protocol without adding autologous plasma.
2. It is induced by pure factors without feeder cells. The culture method is simple and easy to perform. The cell states are basically uniform. Just replenish the liquid according to the recommended process, and there is no need to perform cell counting.
3. When paired with the newly upgraded NK5.0 basal medium, it significantly improves the cell's continuous expansion ability after 7 days of continuous culture, as well as the cell's continuous support ability when expanding from a 2L system to a 3L or even 4L system after 14 days.
4. It can be paired with the research - grade basal medium (NC0102) or the chemically defined - grade basal medium (NC0102.H). The basal medium has been filed with the US FDA's DMF, and the filing number is 37874.
Version Comparison
By collecting 10 samples in different situations, such as hemolytic samples, fresh samples, and cryopreserved samples, and culturing NK cells using the standard version kit and the high-performance version kit respectively, comparing the total number of cells and the positive rate harvested after 14 - 16 days:
The average expansion multiple of the high-performance version after 14 - 16 days of culture reached 202 times, higher than that of the standard version which is 184 times, but the overall fluctuation range of the data is not much different. The average positive rate of the standard version is 71.22% with a large fluctuation range, while the average positive rate of the high-performance version is as high as 92.74%, hardly affected by the initial sample level, and the fluctuation range is extremely low.
Case Display
Special Sample 1: Umbilical cord blood sample transported for 23 hours, collected with a 100mL blood collection bag, with a pure blood volume of only 40mL. Cultured with the high-performance version, finally cultured for 21 days, and harvested a total cell number of 18.9 billion with a positive rate of 88.26% in a 4.5L system.
Special Sample 2: A 62-year-old high-age peripheral blood sample. The sample was transported for 18 hours with a pure blood volume of 50mL. It was cultured using the high-performance version and ultimately cultured for 16 days, harvesting a total of 8.7 billion cells in a 3L system with a positive rate of 98.72%.
Version Selection
Version | Application Scope | Product item number | |
Inoculation blood volume | Item No. | ||
Standard Version | 1. Sample initial positivity rate above 10%; 2. No specific requirement for extremely high positivity rate (above 90%); 3. Can accept special samples with positivity rate below 70%. | 30mL | NC0102+AN0107 |
50mL | NC0102+AN0107-2 | ||
High-Performance Version | 1. Samples with low initial positivity rate or not tested; 2. High demand for extremely high positivity rate (above 90%); 3. Pursuit of extremely high success rate and fault tolerance. As well as the following special circumstances: 1. Samples transported for a long time (transport time exceeding 8 hours); 2. Frozen samples that used non-YOCON mononuclear cell cryopreservation solution; 3. No autologous plasma available for addition. | 30mL | NC0102.F +NC0102+AN0104 |
50mL | NC0102.F +NC0102+AN0104-2 | ||
*All the above-mentioned basal media can be combined with the research-grade serum-free NK cell culture medium (NC0102) or the chemically defined serum-free NK cell culture medium (NC0102.H)
Product Advantages
1. For normal blood samples, the standard version can achieve over 180 - fold cell expansion and a positive rate of over 50% after 14 - 16 days of culture. The high - performance version can achieve over 200 - fold cell expansion and a positive rate of over 85% after 14 - 16 days of culture. The high - performance version can be used for the activation and culture of NK cells in unconventional cord blood or peripheral blood samples, such as blood samples with long - distance transportation time exceeding 8 hours or those with unknown cryopreservation quality, and it supports the culture protocol without adding autologous plasma.
2. It is induced by pure factors without feeder cells. The culture method is simple and easy to perform. The cell states are basically uniform. Just replenish the liquid according to the recommended process, and there is no need to perform cell counting.
3. When paired with the newly upgraded NK5.0 basal medium, it significantly improves the cell's continuous expansion ability after 7 days of continuous culture, as well as the cell's continuous support ability when expanding from a 2L system to a 3L or even 4L system after 14 days.
4. It can be paired with the research - grade basal medium (NC0102) or the chemically defined - grade basal medium (NC0102.H). The basal medium has been filed with the US FDA's DMF, and the filing number is 37874.
Version Comparison
By collecting 10 samples in different situations, such as hemolytic samples, fresh samples, and cryopreserved samples, and culturing NK cells using the standard version kit and the high-performance version kit respectively, comparing the total number of cells and the positive rate harvested after 14 - 16 days:
The average expansion multiple of the high-performance version after 14 - 16 days of culture reached 202 times, higher than that of the standard version which is 184 times, but the overall fluctuation range of the data is not much different. The average positive rate of the standard version is 71.22% with a large fluctuation range, while the average positive rate of the high-performance version is as high as 92.74%, hardly affected by the initial sample level, and the fluctuation range is extremely low.
Case Display
Special Sample 1: Umbilical cord blood sample transported for 23 hours, collected with a 100mL blood collection bag, with a pure blood volume of only 40mL. Cultured with the high-performance version, finally cultured for 21 days, and harvested a total cell number of 18.9 billion with a positive rate of 88.26% in a 4.5L system.
Special Sample 2: A 62-year-old high-age peripheral blood sample. The sample was transported for 18 hours with a pure blood volume of 50mL. It was cultured using the high-performance version and ultimately cultured for 16 days, harvesting a total of 8.7 billion cells in a 3L system with a positive rate of 98.72%.
