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Designated for the cryopreservation of peripheral blood and umbilical cord blood mononuclear cells. The recommended cryopreservation density for peripheral and cord blood mononuclear cells is 3E6~2E7/mL.
It has an excellent protective effect on mononuclear cells in blood samples, and the activated state of cells after resuscitation is basically the same as that of fresh samples.
Product information
Product name | Item No. | Specification | Storage Condition | Grade |
Freezing medium for mononuclear cells | NC1014.1 | 100 mL/ bottle | 2 ～ 8℃ | Chemical Defined |
NC1014.2 | 100 mL/ bottle | 2 ～ 8℃ | Research |
Performance Display
*When mononuclear cells are cryopreserved at three different density gradients, the cell recovery rate is above 70% and the cell viability is above 90% for all gradients.
*After cryopreserving mononuclear cells at three different density gradients, the induced NK cells, upon recovery, exhibited a positivity rate above 85% on Day 7 of culturing and above 90% on Day 14. The cell activation efficiency was consistent with that of fresh samples.
Culturing Examples of Cryopreserved Samples
*Cryopreserved peripheral blood mononuclear cell samples were cultured in a 2-liter system. After 14 days of culturing, a total of 6.48 billion cells were harvested, with a CD3- CD56+ ratio reaching 96.27%.
*Cryopreserved umbilical cord blood mononuclear cell samples were cultured in a 3-liter system. After 18 days of culturing, a total of 9.81 billion cells were harvested, with a CD3- CD56+ ratio reaching 81.24%.
Product information
Product name | Item No. | Specification | Storage Condition | Grade |
Freezing medium for mononuclear cells | NC1014.1 | 100 mL/ bottle | 2 ～ 8℃ | Chemical Defined |
NC1014.2 | 100 mL/ bottle | 2 ～ 8℃ | Research |
Performance Display
*When mononuclear cells are cryopreserved at three different density gradients, the cell recovery rate is above 70% and the cell viability is above 90% for all gradients.
*After cryopreserving mononuclear cells at three different density gradients, the induced NK cells, upon recovery, exhibited a positivity rate above 85% on Day 7 of culturing and above 90% on Day 14. The cell activation efficiency was consistent with that of fresh samples.
Culturing Examples of Cryopreserved Samples
*Cryopreserved peripheral blood mononuclear cell samples were cultured in a 2-liter system. After 14 days of culturing, a total of 6.48 billion cells were harvested, with a CD3- CD56+ ratio reaching 96.27%.
*Cryopreserved umbilical cord blood mononuclear cell samples were cultured in a 3-liter system. After 18 days of culturing, a total of 9.81 billion cells were harvested, with a CD3- CD56+ ratio reaching 81.24%.
