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Product Information
| Product Name | Catalog No. | Product Specification | Storage Condition |
| Bacteria Detection Kit (qPCR Method) | BA0001-50 | The test kit contains 5×BAC reaction solution, enzyme, BAC positive control, BAC internal control, PCR dilution buffer, and 50 tests/kit. | Transport and store at -20℃ Shelf life: 12 months. |
This product is used for the rapid qualitative detection of bacterial contamination in culture media, cell culture materials, and biological products, and test results can be obtained in 2 hours.
It can detect five types of bacteria used for sterility testing in the pharmacopoeia: Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and Escherichia coli. The detection limit is not more than 100 CFU/mL. The detection limits for Pseudomonas aeruginosa and Bacillus subtilis are verified to be 0.5 CFU/mL. The database comparison covers nearly 400 types of bacteria.
Using FAM and ROX two-color fluorescent probes, it can detect bacterial target sequences and internal quality control respectively. It has good specificity and can eliminate false-negative results caused by PCR-inhibiting factors in samples. There is no cross-reaction with fungi, mycoplasma, NK cells, and 293 cells and other human-derived cells.
Performance Verification I: Specificity
*It has been verified that it can detect five kinds of reference bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella, and Escherichia coli) mentioned in the Chinese Pharmacopoeia at a concentration of 100 CFU/mL, and has no cross-reaction with fungi, mycoplasmas, 293 cell DNA, NK cell DNA, and human umbilical cord mesenchymal stem cell DNA.
Performance Verification II: Detection Limit and Sensitivity
*Taking Pseudomonas aeruginosa as an example, using a bacterial liquid concentration of 10-0.05 CFU/mL, the results show that the detection limit of Pseudomonas aeruginosa is 0.5 CFU/mL.
*Taking Salmonella as an example, using a bacterial liquid concentration of 5-0.05 CFU/mL, the results show that the detection limit of Salmonella is 0.5 CFU/mL.
Performance Verification III: Repeatability and Stability Testing
*This test kit was repeatedly produced for 3 batches to test the five types of reference bacteria at a concentration of 100 CFU/mL after extraction. The results are shown in the table below. It can stably detect the five types of reference bacteria at 100 CFU/mL, and the results of the three batches are consistent. The test kit has good reproducibility, with a CV < 10%.
*For other related experimental validations, please refer to the "Technical White Paper" of Yocon Biotechnology's products.
Product Information
| Product Name | Catalog No. | Product Specification | Storage Condition |
| Bacteria Detection Kit (qPCR Method) | BA0001-50 | The test kit contains 5×BAC reaction solution, enzyme, BAC positive control, BAC internal control, PCR dilution buffer, and 50 tests/kit. | Transport and store at -20℃ Shelf life: 12 months. |
This product is used for the rapid qualitative detection of bacterial contamination in culture media, cell culture materials, and biological products, and test results can be obtained in 2 hours.
It can detect five types of bacteria used for sterility testing in the pharmacopoeia: Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and Escherichia coli. The detection limit is not more than 100 CFU/mL. The detection limits for Pseudomonas aeruginosa and Bacillus subtilis are verified to be 0.5 CFU/mL. The database comparison covers nearly 400 types of bacteria.
Using FAM and ROX two-color fluorescent probes, it can detect bacterial target sequences and internal quality control respectively. It has good specificity and can eliminate false-negative results caused by PCR-inhibiting factors in samples. There is no cross-reaction with fungi, mycoplasma, NK cells, and 293 cells and other human-derived cells.
Performance Verification I: Specificity
*It has been verified that it can detect five kinds of reference bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella, and Escherichia coli) mentioned in the Chinese Pharmacopoeia at a concentration of 100 CFU/mL, and has no cross-reaction with fungi, mycoplasmas, 293 cell DNA, NK cell DNA, and human umbilical cord mesenchymal stem cell DNA.
Performance Verification II: Detection Limit and Sensitivity
*Taking Pseudomonas aeruginosa as an example, using a bacterial liquid concentration of 10-0.05 CFU/mL, the results show that the detection limit of Pseudomonas aeruginosa is 0.5 CFU/mL.
*Taking Salmonella as an example, using a bacterial liquid concentration of 5-0.05 CFU/mL, the results show that the detection limit of Salmonella is 0.5 CFU/mL.
Performance Verification III: Repeatability and Stability Testing
*This test kit was repeatedly produced for 3 batches to test the five types of reference bacteria at a concentration of 100 CFU/mL after extraction. The results are shown in the table below. It can stably detect the five types of reference bacteria at 100 CFU/mL, and the results of the three batches are consistent. The test kit has good reproducibility, with a CV < 10%.
*For other related experimental validations, please refer to the "Technical White Paper" of Yocon Biotechnology's products.
